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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide ordinarily contains the epitope acknowledged with the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best final results are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic function. Cyclin E types a complex with and capabilities being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress via S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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